Regulatory

Part:BBa_K4779005:Design

Designed by: Ziqi Mi   Group: iGEM23_Nanjing-BioXstem   (2023-09-27)


UAScit-prm1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

UAScit-prm1 (BBa_K4779005) is a tandem fusion of UAScit and pprm1 promoter (BBa_K1346004). UAScit is obtained through PCR amplification using the S. cerevisiae BY4741 genome as a template, resulting in a 275 bp sequence located upstream of the CIT start codon. The sequence of UAScit was got from reference.

References